ZFIN Image: Shamipour et al., 2019, Fig. S3

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Figure Caption

Fig. S3

Speed Analysis of Bulk F-Actin Polymerization Wave, Related to Figure 2

(A) Fluorescence image of an oocyte expressing Utr-GFP to mark F-actin. Semi-circles indicate the ROIs used for measuring the actin intensity in different distances from the center of the wave. Scale bar, 100 $μm$ . (B) Averaged actin intensity for different distances from the wave center using the semi-circular ROIs as indicated in (A) for the duration of the first cycle (0-50 mpf). The dashed black arrow marks the maxima of each curve, indicating polymerization wave propagation. (C) Kymograph of actin movement along the animal-vegetal (AV) axis of the oocyte as a function of time. White dashed lines trace the bulk actin polymerization waves during the first 3 cleavages. Scale bars, 25 $μm$ (y axis) and 10 min (x axis). (D) Speed of the actin polymerization wave. Box and whiskers, min to max. N = 4 experiments, n = 4 oocytes.

Acknowledgments

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Reprinted from Cell, 177(6), Shamipour, S., Kardos, R., Xue, S.L., Hof, B., Hannezo, E., Heisenberg, C.P., Bulk Actin Dynamics Drive Phase Segregation in Zebrafish Oocytes, 1463-1479.e18, Copyright (2019) with permission from Elsevier. Full text @ Cell