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Fig. S7
Knockdown of rprml impairs normal CHT niche morphogenesis. (A-B) Lateral view of double transgenic Tg(flk:mcherry;fli1:eGFP) embryos microinjected at 1-2 cell-stages with (A) control and (B) rprml morpholinos (MOs). The visualization at 48 hpf was done by live longitudinal confocal microscopy. Double arrows show the girth of the caudal hematopoietic tissue (CHT). Asterisks represent intervascular spaces. The number of embryos with the phenotype is shown as a fraction of the total number of examined embryos, indicated in the top right corner in A-B. Scale bars 50 µm.
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