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Fig. S5
rprml deficiency by MOs results in reduce mpx+- and CD41+- cells during hematopoiesis. Fluorescent microscopy images for: (A-C) mpx+- and (D-F) CD41+ cell populations in transgenics Tg(mpx:GFP) and Tg(CD41:GFP) respectively . (B, E) rprml-MO injections cause reduction in mpx+- and CD41+ blood cells. (C, F) Co-injection rprml-MO and rprml mRNA significantly rescued the rprml morphant phenotype. (Bottom panels) Statistical significance was determined using two-tailed unpaired Student’s t-test. *** P ≤ 0.001, **** P ≤ 0.0001.
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