Figure 6

Figure 6

Activation of the Akt/mTOR pathway in aging zebrafish retinas. (A) Representative western blot showing the protein level of mTOR and p-mTOR in young and aging zebrafish retinas. (B) Densitometric mean and SEM normalized to the corresponding level of the loading control protein beta-actin (***P<0.001, n=3. (C) Representative western blot showing the protein expression level of Akt and p-Akt-T308 and p-Akt-S473 in young and aging zebrafish retinas. (D) Densitometric mean and SEM normalized to the corresponding level of the loading control protein beta-actin (*P<0.05, n=3. (E) Photographs of fluorescent immunostained retina cross-sections showing the localization and relative levels of p-mTOR and p-Akt-T308 in the RGC layers of young and aging zebrafish retinas. All photographs were taken at 40x magnification except rat sections were at 20x magnification and p-Akt-T308 at 60x magnification. Young, 4-6 months old zebrafish; Aging, 19-23-month-old zebrafish; Rat, positive control; GCL, ganglion cell layer.