ZFIN Image: Tang et al., 2019, Fig. 9

Image

Figure Caption

Fig. 9 Effects of exogenous regulation of the Wnt and FGF pathways on HC regeneration at 24 hpa and 48 hpa after neomycin injury.

a1–G3: Regenerating cells labeled with BrdU (red) and HCs labeled with GFP (green). a1–a3: 24 hpa after neomycin damage; A1–A3: 48 hpa after neomycin damage; b1–b3: 24 hpa after addition of BIO; B1–B3: 48 hpa after addition of BIO; c1–c3: 24 hpa after addition of IWR-1; C1–C3: 48 hpa after addition of IWR-1; d1–d3: 24 hpa after addition of SU5402; D1–D3: 48 hpa after addition of SU5402; e1–e3: 24 hpa after addition of bFGF; E1–E3: 48 hpa after addition of bFGF; f1–f3: 24 hpa after addition of BIO+SU5402; F1–F3: 48 hpa after addition of BIO + SU5402; g1–g3: 24 hpa after addition of IWR-1 + bFGF; G1–G3: 48 hpa after addition of IWR-1 + bFGF. h and i: Quantification of the HCs (GFP⁺) (h) and regenerating HCs (BrdU⁺ GFP⁺) (i) after blocking or activating the Wnt and FGF pathways. n = 7 fish per group. ** Indicates p < 0.001, ^# indicates p < 0.05, and error bars indicate the standard error of the mean. Scale bar in G3 = 20 μm for a1–G3

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Exp. Mol. Med.