ZFIN Image: Heston et al., 2017, Fig. 8

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Figure Caption

Fig. 8

A p2A sequence is ineffective in expressing either GFP or FoxP2. a Schematic of virus that utilizes a p2A. In theory, a single gene product should be translated which creates a GFP-p2A-FoxP2 fusion protein that is then self-cleaved into separate GFP and FoxP2 molecules that each carry residual p2A peptides on their C- and N-termini, respectively. bTop panels the GFP virus-injected hemisphere showed high levels of native GFP (green) which was further enhanced using an anti-GFP antibody (red). Lower panels in contrast no GFP, native or enhanced, was visible in the contralateral hemisphere injected with the GFP-p2A-FoxP2 virus. Blue signals reflect DAPI staining. c Exemplar song spectrograms of an adult male tutor (top) and his adult (>100 days) male son (bottom)

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Physiol. A