ZFIN Image: Heston et al., 2017, Fig. 7

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Figure Caption

Fig. 7

Virovek AAV2/5 is effective in transducing Area X neurons, whereas AAV2/9 is not. a Low magnification photomicrograph (left) shows that AAV2/5 can transfect a delimited region of the brain. Some transfection is seen dorsal of the striato-nidopallial border, likely the result of mis-targeting of the injection. Scale bar 500 μm. Higher magnification, right, reveals a high transfection rate. Scale bar 100 μm. b Anti-NeuN antibody signals (red) reveal Area X in left-most panel that is outlined in the middle and right panels. AAV2/9 injection in Area X leads to GFP-positive signals (green, middle panel) in cells that are diffusely scattered around the injection site. Images are merged in the right panel

Acknowledgments

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