U Penn AAV2/1-CB7-GFP retrogradely infects at least one, if not more, Area X afferent nuclei. a High numbers of GFP-positive cells are visible at the injection site in Area X (dotted outline). Scale bar 500 μm. b High numbers of YFP-positive cells (effectively labeled by an anti-GFP antibody) were also identified in LMAN (dotted outline). Scale bar 500 μm. c The near perfect fill of HVC is best explained by retrograde transfection from Area X. Scale bar 500 μm. d Retrograde transfection was further confirmed by expression of GFP-positive fibers in RA, which does not receive input from Area X but does receive input from LMAN and HVC. Scale bar 200 μm
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Full text @ J. Comp. Physiol. A
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