ZFIN Image: Liu et al., 2014, Figure 3

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Figure 3

A dinucleotide repeat (DNR) in the DPYSL2 5′-UTR influences gene expression in in vitro reporter assays: Luciferase results from series of constructs containing LR/HR 3-SNP haplotype plus various DNR in HEK293 cells (A) and primary cortical neurons.* Risk 3-SNP haplotype with 13 DNRs (most common allele in DNR risk alleles) showed ~1.5- to 2-fold decrease in expression compared with construct having Risk 3-SNP haplotype with 11 DNRs in HEK293 (B) and primary cortical neurons (C). (D) these data were verified in HEK293 using semiquantitative expression level of transfected luciferase and endogenous Beta-actin mRNA were determined by reverse-transcription polymerase chain reaction (25 cycles).

Acknowledgments

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