ZFIN Image: Archambault et al., 2019, FIG 1

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FIG 1

C. albicans (C.a.) is more virulent than C. parapsilosis (C.p.) in the zebrafish swimbladder infection model. (A) Zebrafish were infected in the swimbladder at 4 days postfertilization (dpf) with 50 to 100 yeast cells. (B) Candida burden at 24 h postinfection (hpi) quantified from confocal z-projections. Data were pooled from 4 experiments. (C to F) Examples of infected swimbladders in Tg(mpx:mCherry):uwm7Tg zebrafish infected with C. parapsilosis (C and D) or C. albicans (E and F). Depicted are normal appearance of swimbladder (6 hpi) (C), partial swimbladder deflation (24 hpi) (D), complete deflation (24 hpi) (E), and distended swimbladder (24 hpi) (F). Bars, 150 μm. The dotted white line indicates the boundary of the swimbladder. (G) Injected fish were monitored for survival for 4 dpi. Data were pooled from 3 independent experiments. Statistics are described in Materials and Methods (*, P ≤ 0.05; **, P ≤ 0.01). Veh, vehicle.

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