ZFIN Image: Zhang et al., 2019, Fig 7

Image

Figure Caption

Fig 7

Transient overexpression of optn or p62 mRNA promotes GFP-Lc3 recruitment to Mm clusters.

(A) Workflow of the experiments in (B-C). optn or p62 mRNA was injected into the one cell stage of embryos and 2 dpi fixed larvae were used for confocal imaging. The entire CHT region was imaged, as indicated by the black box. (B) Representative confocal micrographs of GFP-Lc3 co-localization with Mm clusters in larvae injected with full length or ΔLIR/ΔUBA(N) deletion mRNAs of optn and p62. The arrowheads indicate the overlap between GFP-Lc3 and Mm clusters. Scale bars, 10 μm. (C) Quantification of the percentage of Mm clusters positive for GFP-Lc3 vesicles. Each dot represents an individual larva (≥18 larvae/group). Data are accumulated from two independent experiments. ns, non-significant, *p<0.05, **P<0.01, *** p<0.001.

Acknowledgments

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