Fig. 7

Fig. 7

Notch signaling acts downstream of primary cilia in regulating hematopoietic stem and progenitor cell (HSPC) specification. a, b Whole-mount in situ hybridization (WISH) result of runx1 expression in control embryos, fsd1 morphants, fli1a:NICD-EGFP plasmid-injected embryos, and fsd1 morphants injected with fli1a:NICD-EGFP plasmid at 26 hpf and 36 hpf. Red arrowheads denote the runx1⁺ cells in the AGM region. c Quantification of runx1⁺ cells in the aorta-gonad-mesonephros (AGM) region in b. Data represent the analysis results of two-way ANOVA–Tukey test for multiple comparisons. Error bars, mean ± s.d., n = 15, 17, 17, 6 embryos. **P < 0.01, ****P < 0.0001. d WISH result of runx1 expression (red arrowheads) in control embryos, fsd1 morphants, hs:NICD-EGFP plasmid injected embryos, and fsd1 morphants injected with hs:NICD-EGFP at 36 hpf. These embryos were heat shocked for 30 min at 42 °C from 20 hpf. e Expression of runx1 (red arrowheads) in control, morphants, and fli1a:NICD-EGFP injected morphants at 36 hpf. Scale bars, 100 µm