ZFIN Image: Rausch et al., 2018, Figure 2

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Figure Caption

Figure 2

CAV1 and CAVIN1 Expression Depend on YAP/TAZ-TEAD Interaction

(A) qPCR analysis of established YAP/TAZ targets. mRNA from Y/T KO and L1/L2 KO HEK293A cells compared to WT HEK293A cells (Figures S2F and S2I). Means ± SD.

(B) qPCR data as in (A), analyzed for the expression of CAV1 and CAVIN1 (Figures S2F and S2I). Means ± SD.

(C) Verteporfin-treated WT HEK293A cells analyzed by qPCR. Inhibition of the interaction between YAP-TEAD reduces expression of caveolar genes. Means ± SD.

(D) Western blots of lysates from Y/T KO cells stably re-expressing either control plasmid, myc-tagged WT, or S94A mutant YAP. l.e., long exposure; s.e., short exposure. Note, re-expression of WT YAP, but not S94A mutant, deficient in TEAD binding, restores expression of caveolar as well as established YAP/TAZ-TEAD target genes, CYR61 and LATS2.

(E and F) Mixed population of Y/T KO cells expressing myc-tagged YAP or empty vector (Figure S3C) or mixed population of Y/T KO cells expressing myc-tagged S94A YAP or empty vector and labeled for myc (red), CAV1 (green), and DAPI (blue; F).

(G and H) Mixed cell culture of Y/T KO cells expressing either vector control or myc-tagged YAP (Figure S3D) or mixed cell culture of Y/T KO cells expressing either vector control or myc-tagged S94A YAP (H). Myc (red), CAVIN1 (green), and DAPI (blue) are shown.

Arrows in (E)–(H) highlight examples of myc-YAP-expressing cells. Scale bars (E)–(H) represent 15 μm. Note, only YAP WT induces expression of CAV1 and CAVIN1.

(I) Dot plot of CAV1 levels from images, as shown in (E) and (F). Each dot represents one cell. Means ± SEM.

(J) Dot plot of CAVIN1 levels from images, as shown in (G) and (H). Each dot represents one cell. Means ± SEM.

(K) Dot plot of CAV1 levels from mixed Y/T KO cell population expressing vector control and TAZ (WT) or TAZ (S51A) labeled for CAV1 and TAZ from images as shown in Figures S3E and S3F.

(L and M) qPCR data from myc-tagged WT or S94A YAP compared to vector control expressing Y/T KO cells (L) and WT or S51A TAZ compared to vector control expressing Y/T KO cells levels (M). Means ± SD.

Acknowledgments

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