Figure 1

Figure 1

YAP/TAZ Are Necessary for Caveolar Protein Expression

(A) Confocal images of wild-type (WT) HEK293A cells labeled for DAPI (blue), YAP/TAZ (red), and CAVEOLIN1 (CAV1) (green).

(B and C) YAP/TAZ KO cells (Y/T KO) (B) and LATS1/2 KO (L1/L2 KO) (C) labeled and imaged as cells in (A).

Scale bars (A–C) represent 30 μm. (A)–(C) are related to Figures S1A–S1C, S1F, and S2A–S2D.

(D) Dot plot of quantified CAV1 levels from images, as shown in (A)–(C). In Y/T KO (red), WT (black), and L1/L2 KO (blue) cells, each dot represents one cell. Means ± SEM.

(E) Dot plot of CAVIN1 levels from images as shown in Figure S1B. Means ± SEM.

(F) Western blots from Y/T KO, WT, and L1/L2 KO HEK293A cells (Figures S2E and S2G). GAPDH and HSP90 serve as loading controls.

(G) PhosTag gel-based western blots probed against YAP from cell lysates as in (F) (Figure S2H).

(H) Mixed cell culture of Y/T KO and WT HEK293A cells were fixed and labeled for YAP/TAZ (red), CAV1 (green), and DAPI (blue). Arrows: examples of Y/T KO cells. Note, cells with no YAP/TAZ signal have low CAV1 signal. Scale bar represents 30 μm.

(I) Close up of cells from red box in (H).

(J) Dot plot of CAV1 levels in mixed cell populations of Y/T KO and WT cells analyzed in images as shown in (H). Each dot represents one cell. Means ± SEM.

(K) Mixed cell population as in (H) labeled for YAP/TAZ (red), CAVIN1 (green), and DAPI (blue). Arrows: examples of Y/T KO cells. Zoomed-out image is in Figure S1D.

(L) Dot plot of CAVIN1 levels in mixed populations of Y/T KO and WT cells carried out on images as shown in (K). Means ± SEM.

(M) Cells as in (H), labeled for YAP (green), CAVEOLIN2 (CAV2) (red), and DAPI (blue). Zoomed-out image is in Figure S1E. Arrows: examples of Y/T KO cells.

Scale bars in (I), (K), and (M) are 15 μm. Further related is Figures S7N–S7R.