Figure 8.

Figure 8.

(A) Section of the heatmap in Figure 5A showing the results for colforsin, dihydrofissinolide, deoxygedunin and carapin-8(9)-ene. (B) Enlargement of the cluster containing gedunin-related compounds (cluster two in Figures 4G and 5B), highlighting deoxygedunin, dihydrofissinolide and carapin-8(9)-ene. Compounds that rescued mbp expression are shown as larger nodes; compounds that did not rescue mbp expression are shown as smaller nodes. (C) (i–x) The inner ear at 4 dpf stained for vcanb. Lateral views; anterior to the left. Scale bar (applies to panels i–x): 50 µm. (i) adgrg6^tb233c/DMSO mutant control. (ii – v) Treatment of adgrg6^tb233c mutants with the compounds at 25 µM indicated was able to rescue the tb233c mutant ear phenotype to variable degrees. (vi) adgrg6^fr24/DMSO mutant control. (vii–x) Treatment of adgrg6^fr24 mutants with colforsin rescued otic vcanb expression in the fr24 allele, whereas treatment with dihydrofissinolide, deoxygedunin and carapin-8(9)-ene was unable to rescue the fr24 ear phenotype. (xi–xiv) Representation of the chemical structure of the four compounds tested. Note the structural similarity between deoxygedunin, dihydrofissinolide and carapin-8(9)-ene.