ZFIN Image: Teixeira Rosa et al., 2019, Fig. 5

Image

Figure Caption

Fig. 5

Midline krt4+ cells cover pharynx and esophagus, and meet peridermal cells invading through P3-P6. (A,B) krt4+ cells cover hyoid arch (hy) and branchial arches 1 to 5 (numbered 1–5), as well as roof and floor of the pharynx and esophagus. Note sharp boundary (arrowhead) between krt4+ esophagus and krt4- lining of the gut. (C,C’,D,D’) krt4+ cells cover the lining of the pharynx (C,C’) and the esophagus (D,D’), resp. Note long cell extensions issuing from the krt4+ cells (E,E’) Absence of krt4+ cells in the gut coincides with single-layered epithelium. (F) At 54 hpf, pouches 3–6 are still free of krt4+ cells. (G,H) Progressive invasion of P3 (arrow) by peridermal cells that meet midline krt4+ cells. (A,B,F) sagittal sections, (C–E’,G,H) cross sections. es: esophagus; g: gut; ph: pharyngeal lumen; TB: toluidine blue staining. Scale bars (A–H) = 50 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.