PUBLICATION

Periderm invasion contributes to epithelial formation in the teleost pharynx

Authors
Teixeira Rosa, J., Oralová, V., Larionova, D., Eisenhoffer, G.T., Eckhard Witten, P., Huysseune, A.
ID
ZDB-PUB-190714-4
Date
2019
Source
Scientific Reports   9: 10082 (Journal)
Registered Authors
Huysseune, Ann
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Endoderm/embryology
  • Gills/anatomy & histology*
  • Gills/embryology
  • Pharynx/anatomy & histology*
  • Pharynx/embryology
  • Zebrafish/anatomy & histology*
  • Zebrafish/embryology*
(all 9)
PubMed
31300674 Full text @ Sci. Rep.
Abstract
The gnathostome pharyngeal cavity functions in food transport and respiration. In amniotes the mouth and nares are the only channels allowing direct contact between internal and external epithelia. In teleost fish, gill slits arise through opening of endodermal pouches and connect the pharynx to the exterior. Using transgenic zebrafish lines, cell tracing, live imaging and different markers, we investigated if pharyngeal openings enable epithelial invasion and how this modifies the pharyngeal epithelium. We conclude that in zebrafish the pharyngeal endoderm becomes overlain by cells with a peridermal phenotype. In a wave starting from pouch 2, peridermal cells from the outer skin layer invade the successive pouches until halfway their depth. Here the peridermal cells connect to a population of cells inside the pharyngeal cavity that express periderm markers, yet do not invade from outside. The latter population expands along the midline from anterior to posterior until the esophagus-gut boundary. Together, our results show a novel role for the periderm as an internal epithelium becomes adapted to function as an external surface.
Genes / Markers
Marker Marker Type Name
mpeg1.1GENEmacrophage expressed 1, tandem duplicate 1
1 - 1 of 1
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Figures
Figure Gallery (6 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c264TgTransgenic Insertion
    fr48TgTransgenic Insertion
      gz7TgTransgenic Insertion
        ha01TgTransgenic Insertion
          zc1044aEtTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            No data available
            Antibodies
            Name Type Antigen Genes Isotypes Host Organism
            Ab2-krtmonoclonal
              IgG1Mouse
              zn-8monoclonalIgG1Mouse
              1 - 2 of 2
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              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              GAL4EFGGAL4
              GFPEFGGFP
              mCherryEFGmCherry
              NTREFGNTR
              TomatoEFGTomato
              1 - 6 of 6
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              Mapping
              No data available