ZFIN Image: Grassini et al., 2019, Fig. 7

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Figure Caption

Fig. 7

The uq^23ks mutant phenotype is first evident at 36 hpf. A: Confocal imaging of Alcama staining on 32 hpf embryos shows both chamber morphology (zoom out) and cardiomyocyte perimeter and circularity (white outlines) indistinguishable between sibling and uq^23ksmutants at 32 hpf. B: Dot plots depicting measurements of perimeter and (C) circularity of cardiomyocytes showing no significant difference (ns) between siblings and mutants at 32 hpf. D: Confocal images of Alcama and F‐actin staining on 36 hpf embryos show cardiomyocytes are smaller and more circular in uq^23ks mutants compared with siblings at 36 hpf. Phalloidin staining shows F‐actin failing to traverse cells and remaining cortical in uq^23ks mutants compared with siblings. E,F: Dot plots showing cell perimeter is decreased (e) and circularity is increased (F) in uq^23ks mutants compared with siblings. G:Fluorescence intensity measurement across short diameter of cells (as depicted in phalloidin staining inset) showing poor correlation between Alcama and F‐actin in siblings but strong correlation in uq^23ks mutants.

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Acknowledgments

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