Fig. 1

Lgl2 expression is upregulated in the retina by 72 dpf and localizes basolaterally in photoreceptor and RPE cells.Immunostaining of transverse retinal sections. (A–D′) Lgl2 expression during retinal development: A,A′, 24 hpf; B,B′, 48 hpf; C,C′, 72 hpf; D,D′, 120 hpf. Lgl2 expression is upregulated by 72 hpf in the RPE (C, arrowhead) and the OPL. Asterisks in C denote expression in the ciliary marginal zone (CMZ). OP, olfactory placode; OPL, outer plexiform layer; PRC, photoreceptor cell layer; LE, lens epithelium. Scale bar: 50 µm. (E–E″) At 3 dpf, Lgl2 (E) localizes basolaterally in the PRC and in the RPE. Lgl2 staining does not overlap with that of Crb2a (E′), which localizes to the subapical region (SAR). Arrowheads in E denote lateral Lgl2 localization in RPE cells. E″ shows merged image. (F–I″) Lgl2 localization at 5 dpf. (F–F″) Co-staining of Lgl2 with Crb2a shows that Lgl2 staining remains basolateral as PRCs mature. (G–H″) Co-staining of Lgl2 (G,H) with WGA (G′) or Zpr1 (H′) illustrates Lgl2 localization in the OPL. (I–I″) Co-staining of Lgl2 (I) with Zpr2 (I′) reveals basolateral expression in the RPE (arrowheads in I″ denote lateral localization). OS, outer segments. Scale bar: 10 µm.