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Fig. 4
Characterization of thraa and l-thrb+ crispants. (A)thraa and l-thrb+ crispants show diverse phenotypes with several degrees of body plan affectations as compared with wild-type at 3 dpf. Various phenotypes were observed due the mosaicism of the mutation. (B,C) Represent the percentage of normal, affected and deceased larvae after sgRNA and Cas9 treatment. (D) DNA sequence of sgRNA target site for CRISPR/Cas9 is denoted in gray, whereas PAM sequence is denoted in blue. Insertion-deletions (indels) are highlighted in yellow.
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Full text @ Front Endocrinol (Lausanne)