ZFIN Image: Dohn et al., 2019, Fig. 3

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Figure Caption

Fig. 3

Loss of nr2f2 alleles in nr2f1a mutants produces stronger overt cardiovascular defects.

(A) Two-color ISH for nr2f1a (blue) and nkx2.5:ZsYellow (red) at the 8s stage. Embryo is flat-mounted with dorsal view and anterior left. (B-E) Lateral views of the nr2f1a^wt; nr2f2^wt, nr2f1a^mut; nr2f2^wt, nr2f1a^mut; nr2f2^het, and nr2f1a^mut; nr2f2^mut embryos. n = 16 overtly WT embryos and n = 32 embryos with edema that were genotyped for the experiment shown. While embryos that have two WT alleles are shown, the other combinations of nr2f1a and nr2f2 alleles, other than nr2f1a^het; nr2f2^mut embryos, were indistinguishable from WT embryos at 48 hpf. 1 of 5 embryos that genotyped as nr2f1a^het; nr2f2^mut embryos was indistinguishable from WT, while 4 of the 5 nr2f1a^het; nr2f2^mut embryos displayed a very small amount of blood pooling on the yolk. However, cardiac defects were not found in these embryos. Arrows indicate edema and blood pooling on the yolk. Arrowheads indicated pericardial edema.

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Acknowledgments

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