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Fig. 8
LR-SI image of a 7-day-old elavl3:GCaMP5g; gad1b:RFP; mitfaw2/w2 zebrafish larva treated with 15 mM PTZ. (a) Integration of the intensity at the location of the RoI is calculated. (b) Intensity change of the RoI noted in (a) plotted as a function of time. LR-SI-non shows higher peak signals relative to the background compared to LR-SI. However, the LR-SI-non results in lower SNRs relative to LR-SI method. The scale bar is 40 μm.
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