ZFIN Image: Turner et al., 2019, Fig. 1

Image

Figure Caption

Fig. 1

The ele mutation is in slbp.

(A-A’) Morphology of 2dpf wildtype and ele mutant embryos (red asterisk indicates heart oedema). (B, B’) Heads of 2dpf wildtype (A) and ele (A’) mutant embryos showing the coloboma phenotype in the mutant (arrowhead in B’). (C-C’) RNAseq mapping plot of SNP homozygosity across Chromosome 14 and 15. (D) Domain organisation of wildtype and mutant Slbp predicted proteins. (E) Dot plots of percentages of embryos showing ele phenotypes in control clutches (blue) and in clutches injected with wildtype slbp^TT-AA-RFP RNA (red). Each dot is the percentage from one of seven independent experiments. Thick black bars = standard deviation; fine black line = mean. (F) Reverse transcriptase polymerase chain reaction (RT-PCR) for slbp and slbp2 at developmental stages indicated. (G, H) Whole mount RNA in-situ hybridization for slbp (H) and slbp2 (I) at developmental stages indicated. Scale bars: (A, A’, H, I) 250μm; (B and B’) 100μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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