Fig. 1

Fig. 1

Influence of genetic background on the proteinuria phenotype. (A) Tg(l-fabp:eGFP-DBP) zebrafish backcrossed on homozygous for either the AB (AB fish) or the nacre (nac^w2) background (nacre fish) as well as heterozygous zebrafish for nacre and AB background were treated with PAN at 46?h post-fertilization (hpf). Graph depicts max. fluorescence of circulating eGFP-DBP in the retinal vessel plexus of the fish eye 96?hpf. (B) Representative images of phenotypes of Tg(l-fabp:eGFP-DBP) zebrafish backcrossed on homozygous for either the AB (AB fish) or the nacre (nac^w2) background (nacre fish) were treated with PAN (4?mg/ml) or DMSO at 46?hpf as indicated. Pictures were taken at 96?hpf. (C) Representative transmission electron microscopy images of the glomerular filtration barrier of Tg(l-fabp:eGFP-DBP) zebrafish backcrossed on homozygous for either the AB or the nacre (nac^w2) background were treated with PAN (4?mg/ml) or DMSO at 46?hpf as indicated. Fish were collected at 120?hpf. Podocyte effacement after PAN treatment was more severe in nacre fish compared to AB fish. ***P<0.001, n.s., non-significant. Scale bars: (B) 500 μm, (C) 500 nm.