Fig. 9
hAPOA-I-mCherry colocalizes with dietary TF-cholesterol in enterocytes of tg(lfabp10:hAPOA-I-mCherry) larvae. Representative images showing hAPOA-I-mCherry and TF-cholesterol colocalization in enterocytes after 3 h (A), 4 h (B), and 6 h (C) of 5% egg yolk feed. Arrowheads: colocalization; n = 3–4, with 3–9 fish per n. A greater percent of TF-cholesterol colocalizes with hAPOA-I-mCherry at 6 h into feeding than at 3 h (means ± SE, 1-way ANOVA of tg(lfabp10:hAPOA-I-mCherry) groups, *P < 0.05) (D). APOA-I, apolipoprotein A-I; hAPOA-I, human APOA-I; L, intestinal lumen; N, nucleus; TF-cholesterol, TopFluor-cholesterol.