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Fig. 3
Effects of ectopic expression of znf76 mRNA in zebrafish embryos Lateral view of the embryos (A–C) and magnified lateral view of the embryos (D–F) at 24 hpf. (A,D) WT zebrafish embryo injected with phenol red dye in distilled water, (B,E) zebrafish embryo injected with 50 pg of znf76 mRNA, and (C,F) zebrafish embryo injected with 100 pg of znf76 mRNA. Microinjection of 50 or 100 pg of znf76 mRNA was performed at the 1-cell stage to induce overexpression. Red arrowheads indicate the specific tissues altered at 24 hpf by 50 or 100 pg of znf76 mRNA injection, compared with the WT; red dotted circles indicate the size of the eyes. NT – neural tube, SC – spinal cord, Hb – hindbrain, Mb – midbrain, E – eye, TB – tail bud, NT – neural tube. Scale bars 100 μm.