ZFIN Image: Young et al., 2019, Fig. 8

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Figure Caption

Fig. 8

Loss of tcf7l1a modifies the cct5^u762 mutant eye phenotype.

(A–J) Lateral views of wildtype (A, F), Ztcf7l1a^-/- mutant (B, G) cct5^U762/u762 mutants (C, H), double cct5^U762/u762/Ztcf7l1a^-/- mutants (D, I) and double cct5^U762/u762/Ztcf7l1a^-/- mutants injected with 0.8 pmol of cct3 morpholino (E, J) at indicated stages. Scale bar = 100 µm. Full data in Supplementary file 10O, single experiment, 36hpf, wt n = 4, Ztcf7l1a^-/- n = 9, cct5^-/-n = 8, cct5/Ztcf7l1a^-/- n = 3; 52hpf, wt n = 8, Ztcf7l1a^-/- n = 8, cct5^-/- n = 4, cct5/Ztcf7l1a^-/- n = 3; 52hpf + 2 pmol tp53 morpholino, wt n = 6, Ztcf7l1a^-/- n = 13, cct5^-/- n = 13, cct5/Ztcf7l1a^-/-n = 12; 52hpf + 0.8 pmol cct3 morpholino, wt n = 12, Ztcf7l1a^-/- n = 12. (K) Eye volume quantification at the indicated timepoints and conditions shown in A–J) (data in Supplementary file 1O). Unpaired t-test. (L–O) Immunostaining detecting phosphohistone3 (PH3, green) in wildtype (L), Ztcf7l1a^-/- (M), cct5^-/- (N), cct5^-/-/Ztcf7l1a^-/- (O) eyes at 32hpf. (P) Plot showing the percentage of PH3 positive cells in the eyes shown in L–O) (data in Supplementary file 1Q) Single experiment, wildtype n = 10, Ztcf7l1a^-/- n = 10, cct5^-/- n = 9, cct5/Ztcf7l1a^-/-n = 10, unpaired t-tests.

Figure Data

Phenotype details

Acknowledgments

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Fig. 8

Fig. 8

Loss of tcf7l1a modifies the cct5u762 mutant eye phenotype.

Loss of tcf7l1a modifies the cct5^u762 mutant eye phenotype.