|
Fig. 2 Wnt5b is required for anisotropic cell shape both apically and basally at the MHBC. (A,B) Live confocal images of 24 ss wild-type embryos co-injected with memGFP and control MO (A) or wnt5b MO (B). (C) Quantification of MHB tissue angle and MHBC cell length (y-axis). Data are represented as mean±s.e.m. from eight independent experiments. (D,E) Representative apical (D) and basal (E) digital slices of control morphants. (F,G) Representative apical (F) and basal (G) digital slices of wnt5b morphants. (H) Quantification of anisotropic cell shape using x:z ratio. MHBC cells are outlined in yellow. Boxplots indicate the 25th and 75th percentiles and the median. Eight independent experiments are represented. Control MO, n=10; wnt5b MO, n=10. ***P<0.005. Arrowheads indicate MHBC and arrows indicate MHB tissue angle. Scale bars: 10 μm.