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Fig. 5 Ppar? inactivation did not induce the premature differentiation of neuronal precursor cells. A?D: GW6471?treatment, pparaa ?LBD injection, pparab ?LBD injection, or pparaa ?LBD and pparab ?LBD coinjection did not induce elval3 expression at 10 hpf or 11 hpf, as examined by in situ hybridization (A,B) and quantified by quantitative real?time polymerase chain reaction (C,D). Note the decreased elval3 expression at 11 hpf in Ppar??deficient embryos. Staging of the embryos is shown in the bottom left corner of each panel in A and B.
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