ZFIN Image: Gutzman et al., 2018, Fig. 4

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Figure Caption

Fig. 4

Fak rescues effects of wnt5b knockdown on basal constriction. (A–C′) Live confocal images of the MHB region in prim-6 stage embryos. Single-cell wild-type embryos were co-injected with mGFP and control MO (A,A′), wnt5b MO (B,B′), or wnt5b MO and FAK^Y397E mRNA (C,C′). (D–F) 3D reconstruction of cells outlined in (A′–C′) using 3D doctor with view of the same cell rotated 45°. (G) Quantification of MHBC gross morphology and basal constriction following FAK^397E mRNA rescue of wnt5b knockdown (n>60 per condition). (H) Model pathway for Wnt5b regulation of basal constriction. Arrowheads indicate MHBC. M, midbrain. H, hindbrain. Scale bars: A–C, 50 µm; A′–C′, 25 µm.

Acknowledgments

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