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Fig. 6
Fibronectin is required for cell-surface Vangl2 expression. (A,C) Zebrafish Vangl2 protein expression in wild type and fn1a/1b morpholino (MO)-injected tailbud-stage embryos without (left) and with (right) DAPI nuclear labeling. Immunofluorescence labeling of Vangl2 in the ectoderm (A) and mesoderm (C). (B,D) Representative plot profiles showing average fluorescence intensities across single ectodermal cells in wild type (n=30 cells, 3 embryos) and fn1a/1b morphant embryos (n=30 cells, 3 embryos) in ectoderm (B) and mesoderm (D). Scale bars: 5 µm.
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