PUBLICATION

Vangl2-dependent regulation of membrane protrusions and directed migration requires a fibronectin extracellular matrix

Authors
Love, A.M., Prince, D.J., Jessen, J.R.
ID
ZDB-PUB-181018-1
Date
2018
Source
Development (Cambridge, England)   145(22): (Journal)
Registered Authors
Jessen, Jason R.
Keywords
Fibronectin, Gastrulation, Migration, Polarity, Protrusion, Vangl2
MeSH Terms
  • Cell Polarity
  • Mutation/genetics
  • Membrane Proteins/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Zebrafish Proteins/metabolism*
  • Cell Surface Extensions/metabolism*
  • Animals
  • Time-Lapse Imaging
  • Cell Movement*
  • Extracellular Matrix/metabolism*
  • Phenotype
  • Fibronectins/metabolism*
(all 12)
PubMed
30327324 Full text @ Development
Abstract
During zebrafish gastrulation the planar cell polarity (PCP) protein Vang-like 2 (Vangl2) regulates polarization of cells engaged in directed migration. However, it is unclear whether Vangl2 influences membrane-protrusive activities in migrating gastrula cells and if these processes require the fibronectin extracellular matrix. We report that Vangl2 modulates formation and polarization of actin-rich filopodia-like and large lamellipodia-like protrusions in ectodermal cells. By contrast, disrupted Glypican4/PCP signaling affects protrusion polarity but not protrusion number or directed migration. Analysis of fluorescent fusion protein expression suggests widespread Vangl2 symmetry in migrating cells but enrichment at membrane domains with developing large protrusions compared to non-protrusive domains. We show the fibronectin extracellular matrix is essential for cell surface Vangl2 expression, membrane-protrusive activity, and directed migration. Manipulation of fibronectin protein levels rescues protrusion and directed migration phenotypes in vangl2 mutant embryos, but is not sufficient to restore PCP and convergence and extension movements. Together, our findings identify distinct roles for Vangl2 and Glypican4/PCP signaling during membrane protrusion formation and demonstrate cell-matrix interactions underlie Vangl2-dependent regulation of protrusive activities in migrating gastrula cells.
Genes / Markers
Figures
Figure Gallery (17 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m119
    		Point Mutation
    m209
      		Point Mutation
      vu7
        		Deficiency
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        fn1aMO1-fn1aMRPHLNO
        fn1bMO1-fn1bMRPHLNO
        fn1bMO2-fn1bMRPHLNO
        prickle1aMO1-prickle1aMRPHLNO
        vangl2MO4-vangl2MRPHLNO
        1 - 5 of 5
        Show
        Fish
        No data available
        Antibodies
        Name Type Antigen Genes Isotypes Host Organism
        Ab2-fnpolyclonal
          		Rabbit
          Ab12-gapdhmonoclonal
            		IgG1Mouse
            1 - 2 of 2
            Show
            Orthology
            No data available
            Engineered Foreign Genes
            No data available
            Mapping
            No data available
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            Citation
            Love, A.M., Prince, D.J., Jessen, J.R. (2018) Vangl2-dependent regulation of membrane protrusions and directed migration requires a fibronectin extracellular matrix. Development (Cambridge, England). 145(22):.