ZFIN Image: López Nadal et al., 2018, Fig. 4

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Figure Caption

Fig. 4

Effect of antibiotic exposure on saponin-immune-stimulation. (A) Percent survival of zebrafish exposed to control (E3), ciprofloxacin (4–9 dpf) (5 ug/L) or oxytetracycline (4–9 dpf) (5 ug/ml) + /- saponin (0.5 mg/ml) from 6 – 9 dpf (n = 100 fish / treatment) (Log-rank Mantel-Cox Test for Chi-square). (B) Representative pictures of the antibiotic/saponin-treated Tg(mpeg1:mCherry-F^ump2/mpx:eGFPⁱ¹¹⁴) fish displaying green neutrophils and red macrophages. (C) Quantification of neutrophils and macrophages in the intestinal area (n = 10 fish/ group) (one way ANOVA Kruskal-Wallis test with Dunn’s Multiple comparison Post-Test, mean ± SEM, ^∗p < 0.05). Two independent experiments were performed and one representative experiment is shown.

Acknowledgments

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