ZFIN Image: Cantù et al., 2018, Fig. S2

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Figure Caption

Fig. S2

bcl9 mutants feature normal early cardiac patterning, but variable cardiac phenotypes in bcl9^Δ29 mutants between 2-3 dpf.

(A-L) bcl9 mutants displayed unchanged nkx2.5 (A-D), myh6/amhc (E-H), and gata4 (I-L) expression at 24 hpf compared to wildtype siblings: lateral and dorsal views, anterior to the left. Scale bar, 250 µm. (M-O) Fluorescent and brightfield (inlets) images of drl:EGFP transgenic wildtype and bcl9^Δ29 mutant embryos at 72 hpf (M,N), lateral views, anterior to the left. The hearts of bcl9^Δ29 are patterned into atria and ventricle. The two chambers are slightly misaligned and the embryos develop cardiac edema (O). (O,P) Whole-mount ISH for vcana reveals expanded expression around the atrio-ventricular canal and in the atrium (asterisks) suggesting a defect in valve formation, ventral views, anterior to the left. Scale bars, 200 µm (M,N), 500 µm (P,Q). Diagram in O depicts mean with s.d. from representative clutch of embryos, genotype confirmed by PCR.

Acknowledgments

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