IMAGE

Fig. S2

ID
ZDB-IMAGE-190108-9
Source
Figures for Cantù et al., 2018
Image
Figure Caption

Fig. S2

bcl9 mutants feature normal early cardiac patterning, but variable cardiac phenotypes in bcl9Δ29 mutants between 2-3 dpf.

(A-L) bcl9 mutants displayed unchanged nkx2.5 (A-D), myh6/amhc (E-H), and gata4 (I-L) expression at 24 hpf compared to wildtype siblings: lateral and dorsal views, anterior to the left. Scale bar, 250 µm. (M-O) Fluorescent and brightfield (inlets) images of drl:EGFP transgenic wildtype and bcl9Δ29 mutant embryos at 72 hpf (M,N), lateral views, anterior to the left. The hearts of bcl9Δ29 are patterned into atria and ventricle. The two chambers are slightly misaligned and the embryos develop cardiac edema (O). (O,P) Whole-mount ISH for vcana reveals expanded expression around the atrio-ventricular canal and in the atrium (asterisks) suggesting a defect in valve formation, ventral views, anterior to the left. Scale bars, 200 µm (M,N), 500 µm (P,Q). Diagram in O depicts mean with s.d. from representative clutch of embryos, genotype confirmed by PCR.

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