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Fig. 3
Antitumor activity of anti-angiogenesis alone and in combination with pemetrexed. On 2dpf, CM-Dil labeled A549 xenograft zebrafish embryos were treated with three anti-angiogenesis alone and in combination with pemetrexed for 3 days. Pemetrexed (P) (2 ng per embryo), bevacizumab (B) (28 ng per embryo) and endostar (E) (11 ng per embryo) were injected into embryos and apatinib (A) (0.5 μg/mL) was dissolved in breeding water. (A) Fluorescent images showing A549 xenograft zebrafish larvae at 5dpf treated with different inhibitors. (B) Quantification of fluorescence intensity of tumor. (C) Tumor growth inhibition rates were calculated according to the fluorescence intensities of tumor. Each bar represents the mean ± SEM. One-way ANOVA followed by Dunnett’s test, *p < 0.05, **p < 0.01, ***p < 0.001 when compared with control group.