|
Fig. 1
PKC overactivation during early development causes mild developmental delay and brain size deficit.
a Lateral views of representative larvae (3?dpf) exposed to DMSO, PMA or PMA+Go6983. Head size was calculated with the boundary shown in yellow dotted line. b Quantitative representation of body length and head size (3?dpf) normalized to DMSO (DMSO n?=?8, PMA n?=?10, PMA+Go6983 20?nM n?=?6, PMA+Go6983 100?nM n?=?10, **p?<?0.01, ***p?<?0.001, one-way ANOVA). c Lateral views of representative larvae (9?dpf) previously exposed to DMSO or PMA. Head size was calculated with the boundary shown in yellow dotted line. d Quantitative representation of body length and head size (9?dpf) normalized to DMSO (DMSO n?=?8, PMA n?=?6, *p?<?0.05, t-test analysis). e Dorsal views of zebrafish brains (3?dpf) visualized by Huc:GFP. Brain region was divided by yellow dotted line. Scale bar, 100??m. f Quantitative representation of different brain regions normalized to DMSO (n?=?16 per group, ***p?<?0.001, t-test analysis). g DAPI staining of zebrafish head sections (3?dpf). Scale bar, 100??m. h Nuclei number in forebrain and midbrain (n?=?6 per group, ***p?<?0.001, t-test)