ZFIN Image: Xu et al., 2018, Fig. 1

Image

Figure Caption

Fig. 1

TBK1/IKK? inhibition augments ?-cell regeneration in a zebrafish model of type 1 diabetes. (A, B, D?H) Bright-field images combined with fluorescent images showing the overall morphology and [Tg(ins:CFP-NTR)^s892; Tg(ins:Kaede)^jh6] expression (green) of larvae at 48 hpa treated with DMSO (A), BX795 (B), BX912 (D), amlexanox (E), AZ-5c (F), AZ-5e (G), and PIAA (H), respectively. While TBK1/IKK?-Is substantially expanded [Tg(ins:CFP-NTR)^s892; Tg(ins:Kaede)^jh6]-expressing cell population (white squares and insets) during regeneration (B and E?H) compared to DMSO (A), the PDPK1 inhibitor BX912 showed minimum effect (D). (C) BX795 is a dual inhibitor of PDPK1 and TBK1/IKK?. (I) Quantification of the number (mean?�?SD) of total regenerated ?-cells at 48 hpa (in A-B and D-H; 4.3?�?1.3 (DMSO), 9.0?�?2.2 (BX795), 4.7?�?1.2 (BX912), 11.0?�?3.4 (amlexanox), 7.4?�?1.4 (AZ-5c), 7.0?�?1.3 (AZ-5e), and 17.7?�?2.4 (PIAA)). Cells in 20 planes of confocal images from 25 individual larvae were counted per condition. ***P?<?0.001.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.