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Fig. 5
Qx partially protects against acute exposure to neomycin. 5dpf larvae were treated for 30 min with neomycin (Neo) 50 μM-400 μM (A,C,E,G) or for 8 hours with Qx 300 μM and Neo was added 30 min before the end of the incubation time (B,D,F,H). Untreated or Qx-treated animals were used as controls. Animals were fixed, stained for otoferlin (red) and phalloidin (green) and analyzed by confocal microscopy. (I) Quantification of the number of hair cells per neuromast after the different treatments represented as mean +/− SEM. One-way ANOVA, Dunnett post test. *p < 0.05, **p < 0.01, ***p < 0.001. Black asterisks compared versus control. Red asterisk compared versus the corresponding Neo-only treatment. (J) Scores for neuromast morphology. Scale bar: 6 μm. Data were taken from at least 20 animals and 3 experiments runs.