Fig. 7
ROS and Hedgehog signalling do not have similar roles during tail development. a DPI treatment at 100??M from 14 to 24 hpf does not affect larval tail morphology (10/10, number of experiments?=?3). Scale bar is 200??m. b shha and ihhb expression in the tail is not affected by DPI treatment at 100??M from 14 to 18 hpf (20/20 for all panels, number of experiments?=?2). c tcf7 and raldh2 expression in the tail bud is not affected by treatment with 20??M cyclopamine from 8 to 16 hpf (20/20 for all panels, number of experiments?=?2). d Loss of Hedgehog signalling does not affect tail formation. Larvae were treated with 20??M cyclopamine from 8 to 10hpf (10/10, number of experiments?=?2). Homozygous smob577 mutants (7/7, number of experiments?=?2) lack zygotic Hedgehog signalling activity. Embryos were manually dechorionated prior to chemical treatment