ZFIN Image: Ellett et al., 2018, Fig. 6

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Figure Caption

Fig. 6

Depletion of the macrophage niche increases conidial destruction during establishment of infection.

(A) Ablation of macrophages decreases conidial viability in the first 24 hpi. (i) and (ii) show representative images of nitroreductase-dependent metronidazole-mediated macrophage ablation following treatment of transgenic embryos (ii) versus untreated control embryos (i). Ablation efficiency is quantified in (S9A Fig). (B) T. marneffei CFU numbers at 24 hpi in macrophage-replete control (Tg(mpeg1:Gal4FF/UAS-E1b:Eco.nfsB-mCherry) negative, treated with 10 mM metronidazole) compared to macrophage-depleted (Tg(mpeg1:Gal4FF/ UAS- E1b:Eco.nfsB-mCherry) positive, treated with 10 mM metronidazole). (C) Ablation of neutrophils increases conidial viability in the first 24 hpi. (i) and (ii) show representative images of Nitroreductase-dependent metronidazole-mediated neutrophil ablation in treated (ii) versus diluent-treated control embryos (i). Ablation efficiency is quantified in (S9B and S9C Fig). (D) T. marneffei CFU numbers at 24 hpi in neutrophil-replete control (Tg(mpx:KalTA4/UAS-E1b:Eco.nfsB-mCherry) negative, treated with 10 mM metronidazole) compared to neutrophil-depleted (Tg(mpx:KalTA4/ UAS- E1b:Eco.nfsB-mCherry) positive, treated with 10 mM metronidazole) Data are mean±SEM, n≥5 embryos/group/experiment, n≥3 experiments.

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Acknowledgments

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