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Fig. S2
Exploring activation depth. a) Schematic of a 5-7 dpf zebrafish showing relative dimensions. b) Successful labeling (red fluorescence) in the head area after irradiating a patch of 50 ?m2, which resulted in an activation area of 150 ?m2 corresponding to a single cell and depth z ? 200 ?m (the boxed area is magnified in the right image, scale bar = 20 ?m). c-d) We also designed an experiment where we could irradiate through fish tissue in the tail zone, but deeper than 200 ?m. We superposed two fish in a crossed position, as shown in the schematic (c), and irradiated the crossed area using this configuration. We could activate areas of 1600 ?m2 passing through tissue length z ? 360 ?m (d) (Scale bar = 80 ?m).