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Fig. 8
Anti-inflammatory effect of the P. parvulus 2.6 ?-glucan in an induced inflammation model of the Tg(mpx:GFP) zebrafish line. The inflammation was induced by cutting off the apical region of the tail. (A) Images of representative larvae treated and untreated with the ?-glucan are depicted. Squares mark the areas of inflammation, and the colors indicate regions (red) with less migration of neutrophils than others (white). (B) The green fluorescence emitted by the neutrophils was quantified in the whole larvae and in their tails regions. The fluorescence values detected during the inflammation procedure were expressed as increased percentages of the values detected at 0 h, and represented as logarithms. Statistical significances between groups are represented by ?P ? 0.05 and ??P ? 0.01.