Fig. 1

Fig. 1

Defective somite segmentation in zebrafish ripply1 mutants. (A) Schematic representation of the ripply1 genomic locus and TALEN target site. The genomic structure of zebrafish ripply1 is composed of four exons. Nucleotides corresponding to the open reading frame (grey), WRPW motif (red), and Ripply homology domain (blue) are indicated. The arrowhead points to the position of the TALEN-target site in the first exon. The nucleotide sequence in the first exon and its translated amino acid and the TALEN targets (left, red; right, green) used in this study are shown below the drawing. In the sud101 allele, a 10-bp deletion induced by TALEN pairs results in a frameshift at the 12th amino acid of Ripply1 and an additional five abnormal amino acids (red) followed by the termination codon. (B) Schematic diagrams of the Ripply1 protein encoded by wild-type and sud101 alleles. The WRPW motif (red) and Ripply homology domain (blue) are shown. In the sud101 allele, five abnormal amino acids due to the frame-shift are shown as an orange box. (C) Lateral views of wild-type, tbx6^−/−, and ripply1^−/− embryos at the 8-somite and 18-somite stages. (D) Representative lateral images of wild-type, tbx6^−/−, and ripply1^−/− embryos at 36 hpf stained with a segment boundary marker, xirp2a/cb1045. Scale bars in C and D represent 100 μm.