ZFIN Image: Guner-Ataman et al., 2018, Fig. 7

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Figure Caption

Fig. 7

Small-Molecule-Mediated Inhibition of the Gdf3 Receptor Alk4 Phenocopies the nkx2.5⁺ Progenitor Specification Defect Observed in tbx1 Mutants

(A–C) Bright-field images of 6 somite stage (ss) wild-type embryos processed by in situ hybridization with an nkx2.5 riboprobe after being treated from 75% epiboly with DMSO (A; n = 18), 20 μM SB-505124 (B; n = 17), or 40 μM SB-505124 (C; n = 20). Dorsal views, anterior up.

(D) Dot plot showing the average sizes of the bilateral nkx2.5 expression domain, normalized to the total embryo area shown in each image, in each experimental group. ^∗∗p < 0.01; ^∗∗∗∗p < 0.0001, as determined by one-way ANOVA followed by Tukey’s multiple comparisons test.

(E–G) Bright-field images of 6 ss wild-type embryos processed by in situ hybridization with a tbx1 riboprobe after being treated with DMSO (E; n = 18), 20 μM SB-505124 (F; n = 18), or 40 μM SB-505124 (G; n = 19) beginning at 75% epiboly. Dorsal views, anterior up.

(H) Dot plot showing the average sizes of the bilateral tbx1 expression domain, normalized to the total embryo area shown in each image, in each experimental group. n.s., nonsignificant as determined by a one-way ANOVA test.

Scale bars, 25 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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