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Fig. 6
C. semilaevis Rspo3 affects mesoderm formation by regulating the Wnt/β-catenin signaling pathway. (A) AB strain D. rerio 24 hpf embryos after treatment with 0.15 μM BIO or 1.67 μM XAV-939, or microinjection with 1000 pg C. semilaevis Rspo3 mRNA per embryo. All embryos are shown oriented with anterior to the left. Scale bar = 200 μm; (B) Abnormality rates of embryos in each group as shown in (A), N is the number of total samples analyzed in each group; (C) WISH analysis of ntl at the gastrula stage in each group; a–d are lateral views, whereas a’–d’ are quarter views. Scale bar = 200 μm; (D) RT-PCR analysis of ntl at the gastrula stage in each group. β-actin is the reference gene. The data are shown as mean ± SD (n = 3, N = 30). The superscript indicates statistical significance (** p < 0.01).
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