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Fig. 5
Overexpression of C. semilaevis Rspo3 affects angiogenesis in D. rerio embryos. (A) Vascular abnormalities induced by overexpression of C. semilaevis Rspo3 mRNA at 36 hpf; a and b show the most heavily impacted transgenic (flila: EGFP) strain D. rerio embryos. Each embryo was injected with 700 pg of C. semilaevis Rspo3 mRNA at 1–4 cell stages; c and d are the control group without injection. The yellow arrowheads indicate different types of vessels, whereas the red asterisks point out the abnormalities in the experimental group. All panels show the embryos oriented with anterior to the left. Scale bar = 100 μm. LDA: lateral dorsal aorta, CCV: common cardinal vein, Se: intersegmental vessel, CV: caudal vein, CA: caudal aorta; (B) Expression analysis of VEGFa at the 50%-epiboly stage and 24 hpf. β-actin is the reference gene. The data are shown as mean ± SD (n = 3, N = 30). Values with different superscripts or an asterisk indicate different statistical significance (* p < 0.05; ns p > 0.05).
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