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Fig. S4
5hmC levels was not affected by high levels of D2HG in transgenic Nes R132H mutant zebrafish.
A. 5hmC levels in NesIDH1wt, Nes R132H and non-transgenic zebrafish embryos were measured using slotblot stained with an 5hmC antibody (quantification of bands on the right panel). Similar results were obtained in three independent experiments one of which is shown below. B. Representative images showing 5-hmC immunostaining in NesIDH1wt, Nes R132H transgenic and non-transgenic zebrafish embryo slices at 3dpf. NT: non-transgenic zebrafish.
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