Fig. 6

Fig. 6

Inducible Etv2 knockdown results in decreased expression of genes associated with lymphangiogenesis as analyzed by in situ hybridization at 56 hpf. (A, E, I) Uninjected larvae illustrate normal flt4, lyve1b and fli1a expression in the PCV. (B, F, J) Larvae injected with photomorph solution and kept in dark show expression pattern similar to uninjected controls. (C, G, K) Larvae injected with photomorph solution and photoactivated at the shield stage (6 hpf) show absent flt4 and lyve1b expression from the major axial vessels. fli1a expression is present in the axial vessels due to partial recovery of Etv2 knockdown phenotype observed at the later stages; however, ISV sprouting is abnormal. (D, H, L) Injected larvae photoactivated at 24 hpf, show decreased flt4 and lyve1b expression (D,H), compared to control larvae. However, note the unaffected pattern of fli1a (L), demonstrating that Etv2 knockdown at 24 hpf specifically affects markers associated with lymphangiogenesis. Analysis for each marker has been repeated at least twice. Number of larvae showing a displayed phenotype out of the total number of larvae is shown in the lower right corner.