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Fig. 1
Determination of pharmacological injury doses—1 nL injection at 3 dpf.(A) Representative images showing pericardial edema (arrows) and remaining yolk reserves (*) presented in a portion of larvae administered adriamycin or puromycin. (B) Five milligrams per milliliter puromycin does not result in edema. Increasing the puromycin dose to 25 mg mL−1 causes edema in >40% of fish from 24 hpi (mean ± SEM). (C) Four milligrams per milliliter adriamycin causes edema in >75% of larvae by 24 hpi (mean ± SEM). SEM, standard error of the mean; dpf, days postfertilization; hpi, hours postinjection.
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