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Fig. 2 Generation of miR-7a-sponge transgenic zebrafish. (A) Cloning of pri-miR-7a and miR7a-SP into LF2.8 expression vectors. Stem-loop structure of premiR-7a, in which mature miR-7a is highlighted in red. (B) Red fluorescent images of C7aSP expression in the entire body of bC7aSP (1, 9 pdf, magnification 40×, scale bars: 200 μm; 2, 4 mpf, magnification 40×, scale bars: 100 mm) and the livers of hC7aSP1 (3, 9 pdf, magnification 40×, scale bars: 200 μm; 4, 4 mpf, magnification 40×, scale bars: 100 mm). (C) Stem-loop RT-qPCR analysis of mature miR-7a of the liver, brain, intestine, and muscle tissues from the three transgenic lines, bC7aSP and hC7aSP1 and 2. **